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cell aggregation chimera

2019-01-30T17:01:22+00:00
  • Aggregation Chimeras: Combining ES Cells, Diploid,

    The three components that can be combined to produce chimeras: diploid embryos, tetraploid embryos, and ES cells. (1) Diploid embryodiploid embryo, (2) diploid embryo–ES cells, (3) diploid embryo–tetraploid embryos, and (4) tetraploid... Embryo–embryo chimeras are made by aggregating two blastomere-stage embryos.

  • Cited by : 73
  • Production of chimeras by aggregation of

    07/09/2006  Usually, ES cell medium is changed daily, and cells are passaged on the second or third day once they reach 70% confluency. For the generation of

  • Cited by : 87
  • Aggregation Chimeras SpringerLink

    In such chimeras, the embryo proper, the amnion, the yolk sac mesoderm, the allantois and the chorionic mesoderm-derived part of the placenta are completely ES cell-derived, whereas the yolk sac endoderm and the trophoblast cell lineages are tetraploid embryo derived (3, 7, 9).

  • Chimera (genetics) - Wikipedia

    OverviewAnimalsPlantsVirusesEthics and legislationSee alsoFurther readingExternal links

    An animal chimera is a single organism that is composed of two or more different populations of genetically distinct cells that originated from different zygotes involved in sexual reproduction. If the different cells have emerged from the same zygote, the organism is called a mosaic. Chimeras are formed from at least four parent cells (two fertilised eggs or early embryos fused together). Each population of cells keeps its own character and the resulting organism is a mixture of tissues. Cases of human chimerism

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  • Production of chimeras by aggregation of

    Additional steps are included to describe the procedures necessary to produce specialized tetraploid chimeras using tetraploid morula ↔ diploid ES cell aggregations. This increasingly popular form of chimera produces embryos of nearly complete ES cell derivation that can be used to speed transgenic production or ask developmental questions.

  • Cited by : 87
  • In-cell Aggregation of a Polyglutamine-containing

    14/12/2007  We have explored the aggregation process of a chimera composed of a globular protein (cellular retinoic acid-binding protein, CRABP) and huntingtin exon 1 with polyglutamine tracts either above (Q53) or below (Q20) the pathological threshold using Escherichia coli cells as a model intracellular environment.

  • Cell migration pathway in the intestinal epithelium:

    The cell migration pathway in the intestinal epithelium of DDK↔C57BL/6JLac mouse chimeras is demonstrated using Dolichos biflorus agglutinin-peroxidase as strain-specific marker. Cell sheets of one genotype extend in relatively straight lines from crypt to villus apex.

  • Cited by : 247
  • In-cell Aggregation of a Polyglutamine-containing

    We have explored the aggregation process of a chimera composed of a globular protein (cellular retinoic acid-binding protein, CRABP) and huntingtin exon 1 with polyglutamine tracts either above (Q53) or below (Q20) the pathological threshold using Escherichia coli cells as a model intracellular environment.

  • Aggregation Chimeras: Combining ES Cells,

    Nagy A, Rossant J. Production of ES-cell aggregation chimeras. In: Joyner A, ed. Gene Targeting: A Practical Approach. Oxford, UK: IRL Press at Oxford University 1999; 177–205. Google Scholar. 12. Lu TY, Markert CL. Manufacture of diploid/tetraploid chimeric mice. Proc Natl Acad Sci USA 1980; 77:6012–6. Google Scholar. 13. Tanaka M, Gertsenstein M, Rossant J, Nagy A. Mash2 acts cell ...

  • Production of Mouse Chimeras by Aggregating

    01/01/2010  The combination of genetic background of the two chimera components and the means of chimera production (blastocyst injection vs. aggregation) both highly influence the efficiency of somatic and germline contribution of the pluripotent cell line. In case of ES cell injection into blastocysts, high germline transmission rates can be achieved by using C57BL/6 host blastocyst. If cells from ...

  • In-cell Aggregation of a Polyglutamine-containing

    chimeras reports with fidelity on in-cell aggregation (35). Moreover, in our E. coli expression system, aggregates can be isolated at different times after induction of synthesis of the chimera

  • Cell migration pathway in the intestinal epithelium:

    The cell migration pathway in the intestinal epithelium of DDK↔C57BL/6JLac mouse chimeras is demonstrated using Dolichos biflorus agglutinin-peroxidase as strain-specific marker. Cell sheets of one genotype extend in relatively straight lines from crypt to villus apex.

  • Generation of Chimeras by Morula Aggregation

    Usually 10-20 ESCs are injected in a blastocyst (67,68). Alternatively, chimeric embryos can be generated by aggregating ES cells with morula (69). The chimeric embryos (usually 5-10) are then...

  • Contribution of Mouse Embryonic Stem Cells and

    Chimeric mice are important tools for investigating embryonic development as they can provide insights into the function of a specific gene, they can trace the origin of the cell lineage, and they can assess the potential of cells. The first chimeric mouse was produced in

  • Int J Dev Biol - Cell mixing during the early

    Two different inbred strain combinations of mouse aggregation chimeras - C3H/HeN (H-2k) x C57BL/6N (H-2b) and C3H/HeN x BALB/cA (H-2d) were used for cell mixing analysis at two points in time - 24 h after aggregation (just prior to transplantation into foster mothers) and 7.5 days post coitum (p.c.). The cell proportion of two H-2 haplotypes at the blastocyst stage was studied using a ...

  • Generation of Chimeric Rhesus Monkeys -

    20/01/2012  Production of Monkey Chimeras by Aggregation of Four-Cell Embryos. We reasoned that the totipotent blastomeres of cleaving embryos should be capable of incorporating foreign blastomeres and forming chimeras. We focused on the four-cell stage based on the evidence that an isolated single blastomere from this stage embryo can implant and develop into a viable rhesus offspring (Chan et

  • Mouse embryonic chimeras: tools for studying

    Embryonic chimeras of the mouse are well-established tools for studying cell lineage and cell potential. They are also a key part of the analysis of complex phenotypes of mutant mice. By combining embryonic stem cell technology, molecularly tagged mutations and sensitive cell lineage markers, chimeras can provide invaluable insights into the tissue-specific requirement and the mode of action ...

  • Production of Mouse Chimeras by Aggregating

    01/01/2010  Currently, there are three distinct methods for generating mouse chimeras: (1) injection of pluripotent cells into the blastocoel cavity of 3.5 dpc (days post coitum) blastocyst stage embryos (), (2) injection of cells under the zona pellucida of eight-cell stage (2.5 dpc) embryos (), and (3) aggregation of cells with eight-cell stage (2.5 dpc) embryos after removal of the zona ().

  • In-cell Aggregation of a Polyglutamine-containing

    chimeras reports with fidelity on in-cell aggregation (35). Moreover, in our E. coli expression system, aggregates can be isolated at different times after induction of synthesis of the chimera

  • Red blood cell selection in chimeric mice -

    Starch gel electrophoresis of a large number of tissues and organs from C3H in equilibrium C57BL aggregation chimeras suggests that the C57BL red blood cell population predominates, whereas for other tissues the proportions of the two components are more nearly equal. A similar predominance of (C57BL X C3H)F1 red blood cells is seen in (C57BL X C3H)F1 in equilibrium Recessive chimeras ...

  • Chimera (genetics) - Infogalactic: the planetary

    Two- to eight-cell-stage embryos are competent for making chimeras, since at these stages of development, the cells in the embryos are not yet committed to give rise to any particular cell lineage, and could give rise to the inner cell mass or the trophectoderm. In the case where two diploid eight-cell-stage embryos are used to make a chimera, chimerism can be later found in the epiblast ...

  • In-cell Aggregation of a Polyglutamine-containing

    In-cell Aggregation of a Polyglutamine-containing Chimera Is a Multistep Process Initiated by the Flanking Sequence * Zoya Ignatova ‡ § 1, Ashwani K. Thakur ¶, Ronald Wetzel ¶ and Lila M. Gierasch § 2 ‡ Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried 82152, Germany, the § Departments of Biochemistry Molecular Biology and Chemistry, University of Massachusetts ...

  • The effects of embryo stage and cell number on the ...

    The aim of the present study was to test whether 4-cell stage embryos in 4-cell diploid[harr ]8-cell diploid aggregates contributed equally to all lineages present in the E12.5 conceptus. These chimaeras were compared with those produced from standard aggregates of two whole 8-cell embryos and aggregates of half an 8-cell embryo with a whole 8-cell embryo. As expected, the overall contribution ...

  • Mouse embryonic chimeras: tools for studying

    Embryonic chimeras of the mouse are well-established tools for studying cell lineage and cell potential. They are also a key part of the analysis of complex phenotypes of mutant mice. By combining embryonic stem cell technology, molecularly tagged mutations and sensitive cell lineage markers, chimeras can provide invaluable insights into the tissue-specific requirement and the mode of action ...

  • Generation of chimeric rhesus monkeys — Oregon

    Cell Aggregation Medicine Life Sciences. View full fingerprint Cite this. APA; Standard; Harvard ; Vancouver ... These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.", author = "Masahito Tachibana and Michelle Sparman and Cathy Ramsey and Hong Ma and Lee, {Hyo Sang} and Penedo, {Maria ...

  • Efficient Generation of Germ Line Transmitting

    22/06/2010  As shown above, we were able to generate 100% ES cell derived chimeras (as judged by coat colour) using these culture conditions and aggregation method with more 100% by coat colour animals obtained with VGB6-cultured ES cells than with KOSR+2i-cultured ES cells (respectively 2.9 and 1.9 on average per clone, or 19.6% and 11.4% of live born pups, among those clones giving 100% ES cell

  • Multistep process of FUS aggregation in the cell

    Aggregation of mislocalized FUS into non-amyloid inclusions is believed to be pivotal in the development of cell dysfunction, but the mechanism of their formation is unclear. Using transient expression of a panel of deletion and chimeric FUS variants in various cultured cells, we demonstrated that FUS accumulating in the cytoplasm nucleates a novel type of RNA granules, FUS granules (FGs ...

  • Aggregation Chimeras (ES Cell–Embryo) - Lobe -

    One approach for generating transgenic mice from ES cell lines is to aggregate ES cells with morula‐stage embryos to generate chimeric mice. The chimeras are then bred to generate transgenic offspring. This method offers a simpler and less expensive alternative to the method of ES cell injection into blastocyst embryos.

  • ES Cell Aggregation (Mice) The University of

    ES Cell Aggregation (Mice) Information on aggregation of targeted Embryonic Stem (ES) cells to generate transgenic mice . Generation of chimeras through standard ES injection may be challenging for some lines. An alternative method to develop chimeric animals is to use either diploid or tetraploid ES aggregation. These services are only offered if traditional ES cell injection techniques have ...

  • Production of chimeras by aggregation of

    Although this has not been rigorously tested, it is commonly believed that ES cell ↔ morula aggregation chimeras will have similar behaviors. In recent years, the use of 129B6 hybrid ES cells has proven particularly efficient for the production of tetraploid ↔ diploid chimeras 6, 32, 33. Pups derived from this technique show essentially wild-type characteristics, although they tend to be ...

  • Aggregation chimeras: Combining es cells, diploid,

    During the past 40 years, mouse chimeras have served as invaluable tools for studying not only genetics but also embryonic development, and the path from undifferentiated cell populations to fully committed functional cell types. This chapter gives a description of the early events of cell commitment and differentiation in the pre-and postimplantation-stage embryo. Next, a discussion follows ...

  • CHIMERA signification, définition dans le dictionnaire ...

    chimera définition, signification, ce qu'est chimera: 1. in Greek mythology (= ancient stories), a creature with a lion's head, a goat's body, and a. En savoir plus.

  • Fluorescent transgenic mice suitable for multi-color ...

    Because of this superior nature, these lines are ideal for the generation of multicolored aggregation chimeras that can be used to study cell-cell interactions and lineage analyses in living embryos/ organs, where the transgenes can be detected and the clonal origin of a given cell population easily traced by its distinct fluorescence. In this study, to verify if Tg fluorescent mice generated ...

  • Chimera (genetics) Project Gutenberg Self

    Graft chimeras. The distinction between sectorial, mericlinal and periclinal chimeras are widely used. [33] [34] Structure Plants. There are a variety of combinations that can give rise to a successful chimera mouse and — according to the goal of the experiment — an appropriate cell and embryo combination can be picked; they are generally but not limited to diploid embryo and ES cells ...

  • Contribution of rat embryonic stem cells to

    In this study, we used 8‐cell embryo aggregation, 8‐cell embryo injection, and blastocyst injection to generate interspecies chimeric mice using rat embryonic stem (ES ) cells and then investigated the differences in the contribution rate of the rat ES cells. The rate of chimeric mouse generation was the highest using blastocyst injection, followed in order by 8‐cell embryo injection and ...

  • Aggresomes, inclusion bodies and protein

    In this model, aggregation is seeded at a single or limited number of nucleation sites. Because the addition of monomer to existing aggregates is strongly favoured by the thermodynamic stability of the aggregate, a ‘founder’ aggregate will tend to dominate and the number of aggregation foci or inclusion bodies in the cell will be limited ...

  • Efficient Generation of Germ Line Transmitting

    22/06/2010  As shown above, we were able to generate 100% ES cell derived chimeras (as judged by coat colour) using these culture conditions and aggregation method with more 100% by coat colour animals obtained with VGB6-cultured ES cells than with KOSR+2i-cultured ES cells (respectively 2.9 and 1.9 on average per clone, or 19.6% and 11.4% of live born pups, among those clones giving 100% ES cell

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